+--137 lines: begin model-------------------------------------------------------------------------------------------------------------------------------------------------------------------
  p6   6e-10/BIMOL*ERKpp_RAF1_FB  # (hyper)phosphorylation rate 
  q6   3e-4                       # de(hyper)phosphorylation rate 


  # -- ERK_pp reporters activation/deactivation:
  #
  a0_ekar3  3e-9                                                                                                                                                                            
  d0_ekar3  1e-3
  #
  a0_erktr  1e-9                                                                                                                                                                            
  d0_erktr  2e-3

end parameters


begin molecule types
+--286 lines: # EGFR, epidermal growth factor receptor:-------------------------------------------------------------------------------------------------------------------------------------

end reaction rules


begin observables

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  Molecules  EGFR_act              EGFR(egf~A)
  Molecules  EGFR_act_SOS_act_cplx EGFR(egf~A,sos!1).SOS(S~U,rem!?,egfr!1)
  Molecules  RAS_act               RAS(g~GTP)
# Molecules  RasGAP_RAS_cplx       RasGAP().RAS()
# Molecules  SOS_act               SOS(S~U)
# Molecules  RAS_act_free          RAS(sos,g~GTP)
  Molecules  RAF_act               RAF(S~A)
# Molecules  MEK_Thr292_p          MEK(T292~P!?)
  Molecules  MEK_act               MEK(S~PP)
  Molecules  ERK_act               ERK(S~PP)
# Molecules  EKAR3_act             EKAR3(act~A)
# Molecules  ERKTR_act             ERKTR(act~A)
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end observables

end model


+-- 25 lines: begin actions-----------------------------------------------------------------------------------------------------------------------------------------------------------------

+--137 lines: begin model-------------------------------------------------------------------------------------------------------------------------------------------------------------------
  p6   6e-10/BIMOL*ERKpp_RAF1_FB  # (hyper)phosphorylation rate 
  q6   3e-4                       # de(hyper)phosphorylation rate 


  # -- ERK_pp reporters activation/deactivation:
  #
  a0_ekar3  3e-9/BIMOL # <-- corrected line (1/BIMOL scaling factor added 29-Aug-2017 by WSH)                                                                                               
  d0_ekar3  1e-3
  #
  a0_erktr  1e-9/BIMOL # <-- corrected line (1/BIMOL scaling factor added 29-Aug-2017 by WSH)                                                                                               
  d0_erktr  2e-3

end parameters


begin molecule types
+--286 lines: # EGFR, epidermal growth factor receptor:-------------------------------------------------------------------------------------------------------------------------------------

end reaction rules


begin observables

  # -- Selection of observables:                                                                                                                                                            
  #                                                                                                                                                                                         
  Molecules  EGFR_act              EGFR(egf~A)
  Molecules  EGFR_act_SOS_act_cplx EGFR(egf~A,sos!1).SOS(S~U,rem!?,egfr!1)
  Molecules  RAS_act               RAS(g~GTP)
# Molecules  RasGAP_RAS_cplx       RasGAP().RAS()
# Molecules  SOS_act               SOS(S~U)
# Molecules  RAS_act_free          RAS(sos,g~GTP)
  Molecules  RAF_act               RAF(S~A)
# Molecules  MEK_Thr292_p          MEK(T292~P!?)
  Molecules  MEK_act               MEK(S~PP)
  Molecules  ERK_act               ERK(S~PP)
# Molecules  EKAR3_act             EKAR3(act~A)
# Molecules  ERKTR_act             ERKTR(act~A)
                                                                                                                                                                                            
  # -- All observables:                                                                                                                                                                     
  #                                                                                                                                                                                         
  Molecules S0  EGFR(egf~I,sos)                                                                                                                                                             
  Molecules S1  RAS(g~GDP,sos)                                                                                                                                                              
  Molecules S2  RAS(g~GTP,sos)                                                                                                                                                              
  Molecules S3  SOS(S~U,egfr,rem)                                                                                                                                                           
  Molecules S4  RasGAP(ras)                                                                                                                                                                 
  Molecules S5  RAF(S~I)                                                                                                                                                                    
  Molecules S6  MEK(S~U,T292~U)                                                                                                                                                             
  Molecules S7  ERK(S~U)                                                                                                                                                                    
  Molecules S8  EKAR3(act~I)                                                                                                                                                                
  Molecules S9  ERKTR(act~I)                                                                                                                                                                
  Molecules S10 EGFR(egf~A,sos)                                                                                                                                                             
  Molecules S11 RAS(g~GTP!1,sos).RasGAP(ras!1)                                                                                                                                              
  Molecules S12 RAF(S~A)                                                                                                                                                                    
  Molecules S13 EGFR(egf~A,sos!1).SOS(S~U,egfr!1,rem)                                                                                                                                       
  Molecules S14 RAS(g~GDP!1,sos).RasGAP(ras!1)                                                                                                                                              
  Molecules S15 MEK(S~P,T292~U)                                                                                                                                                             
  Molecules S16 EGFR(egf~I,sos!1).SOS(S~U,egfr!1,rem)                                                                                                                                       
  Molecules S17 EGFR(egf~A,sos!1).RAS(g~GTP,sos!2).SOS(S~U,egfr!1,rem!2)                                                                                                                    
  Molecules S18 EGFR(egf~A,sos!1).RAS(g~GDP,sos!2).SOS(S~U,egfr!1,rem!2)                                                                                                                    
  Molecules S19 MEK(S~PP,T292~U)                                                                                                                                                            
  Molecules S20 EGFR(egf~I,sos!1).RAS(g~GTP,sos!2).SOS(S~U,egfr!1,rem!2)                                                                                                                    
  Molecules S21 EGFR(egf~I,sos!1).RAS(g~GDP,sos!2).SOS(S~U,egfr!1,rem!2)                                                                                                                    
  Molecules S22 ERK(S~P)                                                                                                                                                                    
  Molecules S23 ERK(S~PP)                                                                                                                                                                   
  Molecules S24 SOS(S~P,egfr,rem)                                                                                                                                                           
  Molecules S25 MEK(S~U,T292~P)                                                                                                                                                             
  Molecules S26 MEK(S~P,T292~P)                                                                                                                                                             
  Molecules S27 MEK(S~PP,T292~P)                                                                                                                                                            
  Molecules S28 RAF(S~P)                                                                                                                                                                    
  Molecules S29 EKAR3(act~A)                                                                                                                                                                
  Molecules S30 ERKTR(act~A)                                                                                                                                                                
  Molecules S31 SOS(S~PP,egfr,rem)                                                                                                                                                          
  Molecules S32 SOS(S~PPP,egfr,rem)                                                                                                                                                         
  Molecules S33 SOS(S~PPPP,egfr,rem)                                                                                                                                                        

end observables

end model


+-- 25 lines: begin actions-----------------------------------------------------------------------------------------------------------------------------------------------------------------