ShuttleTracker
of fluorescent reporters in living cells
ShuttleTracker can detect stained cell nuclei, generate corresponding annular perinuclear extensions, quantify geometric and photometric properties of detected objects, and perform frame-to-frame nuclei tracking. Parameters of the image processing algorithms can be changed manually and the effects of these changes can be seen nearly immediately overlaid on currently displayed microscopic images. A key capability of ShuttleTracker is that nuclear contours and tracks can be generated automatically and then corrected manually.
Example session
Downloads
ShuttleTracker is distributed under GNU GPL v3.0.
The latest ShuttleTracker version is 1.0.0.
Source code
- C++ source code: shuttletracker-1.0.0-source.tar.gz
tar xfz shuttletracker-VERSION-source.tar.gz cd shuttletracker-VERSION-source mkdir build && cd build cmake .. -DCMAKE_BUILD_TYPE=Release -DCMAKE_INSTALL_PREFIX=/opt/local/ make -j4 && make install
Stand-alone binary executables
- Mac application bundle (zipped "app"): shuttletracker-1.0.0-x86_64-darwin16.zip
SECURITY: MD5 sum of the downloadable ZIP archive can be checked in Terminal:
- Windows 64-bit installer: shuttletracker-1.0.0-x86_win64-setup.exe
SECURITY: The downloadable installer is not signed with a certificate (so in windows terms it comes from an "unknown publisher") but its integrity can be checked manually in command line using a Windows pre-installed utility:
Containers
- A docker-based recipe for a Singularity container: shuttletracker-1.0.0-singularity.recipe
singularity build shuttletracker.simg shuttletracker-1.0.0-singularity.recipe
LAUNCHING the simulator within a container (in terminal):
singularity exec shuttletracker-1.0.0.simg /opt/local/bin/ShuttleTracker
Documentation
- User's manual: shuttletracker-1.0.0-manual.pdf
Example images
- An archive with example ShuttleTracker files: shuttletracker-1.0.0-examples.zip
Contact
The lead software developer is Marek Kochańczyk. For both user's and developer's queries please use the following e-mail address: shuttletracker.software@gmail.com