ShuttleTracker
of fluorescent reporters
ShuttleTracker can detect stained cell nuclei, generate corresponding annular perinuclear extensions, quantify geometric and photometric properties of detected objects, and perform frame-to-frame nuclei tracking. Parameters of the image processing algorithms can be changed manually and the effects of these changes can be seen nearly immediately overlaid on currently displayed microscopic images. A key capability of ShuttleTracker is that nuclear contours and tracks can be generated automatically and then corrected manually. The tool is scriptable.
The latest ShuttleTracker version is 1.3.0.
Stand-alone binary executables for Windows and Mac
- Windows 64-bit installer: TODO
SECURITY: The downloadable installer is not signed with a certificate (so in windows terms it comes from an "unknown publisher") but its integrity can be checked manually in command line using a Windows pre-installed utility:
- macOS application bundle (zipped "app"): TODO
SECURITY: MD5 sum of the downloadable ZIP archive can be checked in Terminal:
Containers for Linux
- A docker-based recipe for a Singularity container: shuttletracker-1.3.0-singularity.recipe
singularity build shuttletracker.simg shuttletracker-1.3.0-singularity.recipe
LAUNCHING the simulator within a container (in terminal):
singularity exec shuttletracker.simg /opt/local/bin/ShuttleTracker
Source code
ShuttleTracker source code is distributed under GNU GPL v3.0.
- C++ source code: shuttletracker-1.3.0-source.tar.gz
tar xfz shuttletracker-1.3.0-source.tar.gz cd shuttletracker-1.3.0-source mkdir build && cd build cmake .. -DCMAKE_BUILD_TYPE=Release -DCMAKE_INSTALL_PREFIX=/opt/local/ make -j4 && make install
Documentation
- User's manual: shuttletracker-1.3.0-manual.pdf
- An archive with tutorial instructions and images: TODO
Contact
The lead software developer is Marek Kochańczyk. For both user's and developer's queries please use the following e-mail address: shuttletracker.software@gmail.com